Samantha Stone Sandbox

This is the 3FPB Membrane Protein.

Its structure consists of ATP, adenosine 5'-(beta,gamma-methylene)triphosphate (AMPPCP), and cyclopiazonic acid (CZA). A metal (Mg or Mn) must react in the ATP binding site for CZA to bind and react. This occurs at the M1 helix. Knowing the structure of the binding site allows for drugs that can be designed to target this ATPase.

Full Molecule

Membrane Spanning

Mg II ATP Binding Site

Mg II CZA Binding Site

Mn II Binding Site

Mn II ATP Binding Site

Mn II CZA Binding Site

The following picture shows the M1-M10 helices that are involved sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA). The M1 helix has a kink that is a part of all P-type ATPases. This kink is involved in cytoplasmic cation access pathway. The metal ion needed for binding can also be seen.

http://www.jbc.org/content/284/20/13513/F1.large.jpg